Open AccessDeep three-photon imaging of the brain in intact adult zebrafish
Author(s) -
Dawnis M. Chow,
David Sinefeld,
Kristine E. Kolkman,
Dimitre G. Ouzounov,
Najva Akbari,
Rose L. Tatarsky,
Andrew H. Bass,
Chris Xu,
Joseph R. Fetcho
Publication year - 2020
Publication title -
nature methods
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 19.469
H-Index - 318
eISSN - 1548-7105
pISSN - 1548-7091
DOI - 10.1038/s41592-020-0819-7
Subject(s) - zebrafish , vertebrate , neuroscience , cerebrum , biology , two photon excitation microscopy , optical imaging , neuroimaging , cerebellum , optic tectum , preclinical imaging , tectum , anatomy , central nervous system , midbrain , fluorescence , in vivo , gene , optics , physics , biochemistry , microbiology and biotechnology
Behaviors emerge from activity throughout the brain, but noninvasive optical access in adult vertebrate brains is limited. We show that three-photon (3P) imaging through the head of intact adult zebrafish allows structural and functional imaging at cellular resolution throughout the telencephalon and deep into the cerebellum and optic tectum. With 3P imaging, considerable portions of the brain become noninvasively accessible from embryo to sexually mature adult in a vertebrate model.