Open AccessChemogenetic control of gene expression and cell signaling with antiviral drugs
Author(s) -
Elliot P. Tague,
Hannah L. Dotson,
Shan N. Tunney,
D. Christopher Sloas,
John T. Ngo
Publication year - 2018
Publication title -
nature methods
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 19.469
H-Index - 318
eISSN - 1548-7105
pISSN - 1548-7091
DOI - 10.1038/s41592-018-0042-y
Subject(s) - protease , transmembrane protein , microbiology and biotechnology , biology , ns3 , cell , gene expression , gene , ligand (biochemistry) , transcription factor , transcription (linguistics) , computational biology , signal transduction , biochemistry , enzyme , receptor , linguistics , philosophy
We developed a method in which the NS3 cis-protease from hepatitis C virus can be used as a ligand-inducible connection to control the function and localization of engineered proteins in mammalian cells. To demonstrate the versatility of this approach, we designed drug-sensitive transcription factors and transmembrane signaling proteins, the activities of which can be tightly and reversibly controlled through the use of clinically tested antiviral protease inhibitors.