Open AccessHigh-speed volumetric imaging of neuronal activity in freely moving rodents
Author(s) -
Oliver Skocek,
Tobias Nöbauer,
Lukas Weilguny,
Francisca Martínez Traub,
Chuying Naomi Xia,
Maxim I. Molodtsov,
Abhinav Grama,
Masahito Yamagata,
Daniel Aharoni,
David D. Cox,
Peyman Golshani,
Alipasha Vaziri
Publication year - 2018
Publication title -
nature methods
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 19.469
H-Index - 318
eISSN - 1548-7105
pISSN - 1548-7091
DOI - 10.1038/s41592-018-0008-0
Subject(s) - calcium imaging , premovement neuronal activity , microscope , hippocampus , signal (programming language) , biomedical engineering , materials science , optics , calcium , computer science , neuroscience , physics , biology , medicine , programming language , metallurgy
Thus far, optical recording of neuronal activity in freely behaving animals has been limited to a thin axial range. We present a head-mounted miniaturized light-field microscope (MiniLFM) capable of capturing neuronal network activity within a volume of 700 × 600 × 360 µm 3 at 16 Hz in the hippocampus of freely moving mice. We demonstrate that neurons separated by as little as ~15 µm and at depths up to 360 µm can be discriminated.