Open AccessDistinct microbial and immune niches of the human colon
Author(s) -
Kylie R. James,
Tomás Gomes,
Rasa Elmentaite,
Nitin Kumar,
Emily L. Gulliver,
Hamish W. King,
Mark Stares,
Bethany Bareham,
John R. Ferdinand,
Velislava Petrova,
Krzysztof Polański,
Samuel C. Forster,
Lorna B. Jarvis,
Ondřej Suchánek,
Sarah Howlett,
Louisa K. James,
Joanne L. Jones,
Kerstin B. Meyer,
Menna R. Clatworthy,
Kourosh SaebParsy,
Trevor D. Lawley,
Sarah A. Teichmann
Publication year - 2020
Publication title -
nature immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.074
H-Index - 388
eISSN - 1529-2916
pISSN - 1529-2908
DOI - 10.1038/s41590-020-0602-z
Subject(s) - immune system , biology , cecum , immunology , mesenteric lymph nodes , adaptation (eye) , lymphatic system , microbiology and biotechnology , ecology , neuroscience
Gastrointestinal microbiota and immune cells interact closely and display regional specificity; however, little is known about how these communities differ with location. Here, we simultaneously assess microbiota and single immune cells across the healthy, adult human colon, with paired characterization of immune cells in the mesenteric lymph nodes, to delineate colonic immune niches at steady state. We describe distinct helper T cell activation and migration profiles along the colon and characterize the transcriptional adaptation trajectory of regulatory T cells between lymphoid tissue and colon. Finally, we show increasing B cell accumulation, clonal expansion and mutational frequency from the cecum to the sigmoid colon and link this to the increasing number of reactive bacterial species.