Open AccessGenetically encoded protein sulfation in mammalian cells
Author(s) -
James S. Italia,
Jennifer C. Peeler,
Christen Hillenbrand,
Christopher Latour,
Eranthie Weerapana,
Abhishek Chatterjee
Publication year - 2020
Publication title -
nature chemical biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.412
H-Index - 216
eISSN - 1552-4469
pISSN - 1552-4450
DOI - 10.1038/s41589-020-0493-1
Subject(s) - sulfation , tyrosine , escherichia coli , sulfotransferase , recombinant dna , transfer rna , biochemistry , genetic code , biology , cofactor , gene , microbiology and biotechnology , chemistry , enzyme , rna
Tyrosine sulfation is an important post-translational modification found in higher eukaryotes. Here we report an engineered tyrosyl-tRNA synthetase/tRNA pair that co-translationally incorporates O-sulfotyrosine in response to UAG codons in Escherichia coli and mammalian cells. This platform enables recombinant expression of eukaryotic proteins homogeneously sulfated at chosen sites, which was demonstrated by expressing human heparin cofactor II in mammalian cells in different states of sulfation.