Open AccessCompact RNA editors with small Cas13 proteins
Author(s) -
Soumya Kannan,
Han Altae-Tran,
Xin Jin,
Victoria J. Madigan,
Rachel Oshiro,
Kira S. Makarova,
Eugene V. Koonin,
Feng Zhang
Publication year - 2021
Publication title -
nature biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 15.358
H-Index - 445
eISSN - 1546-1696
pISSN - 1087-0156
DOI - 10.1038/s41587-021-01030-2
Subject(s) - rna , crispr , gene knockdown , rna editing , computational biology , cytosine , biology , dna , microbiology and biotechnology , genetics , gene
CRISPR-Cas13 systems have been developed for precise RNA editing, and can potentially be used therapeutically when temporary changes are desirable or when DNA editing is challenging. We have identified and characterized an ultrasmall family of Cas13b proteins-Cas13bt-that can mediate mammalian transcript knockdown. We have engineered compact variants of REPAIR and RESCUE RNA editors by functionalizing Cas13bt with adenosine and cytosine deaminase domains, and demonstrated packaging of the editors within a single adeno-associated virus.