Open AccessDynamin-dependent vesicle twist at the final stage of clathrin-mediated endocytosis
Author(s) -
Xiaodong Cheng,
Kuangcai Chen,
Bin Dong,
Meek Yang,
Seth L. Filbrun,
Yong Myoung,
Teng-Xiang Huang,
Yan Gu,
Gufeng Wang,
Ning Fang
Publication year - 2021
Publication title -
nature cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 11.38
H-Index - 369
eISSN - 1476-4679
pISSN - 1465-7392
DOI - 10.1038/s41556-021-00713-x
Subject(s) - dynamin , endocytosis , vesicle , microbiology and biotechnology , clathrin , gtp' , receptor mediated endocytosis , bulk endocytosis , biophysics , chemistry , biology , cell , biochemistry , membrane , enzyme
Dynamin has an important role in clathrin-mediated endocytosis by cutting the neck of nascent vesicles from the cell membrane. Here, using gold nanorods as cargos to image dynamin action during live clathrin-mediated endocytosis, we show that, near the peak of dynamin accumulation, the cargo-containing vesicles always exhibit abrupt, right-handed rotations that finish in a short time (~0.28 s). The large and quick twist, herein named the super twist, is the result of the coordinated dynamin helix action upon GTP hydrolysis. After the super twist, the rotational freedom of the vesicle increases substantially, accompanied by simultaneous or delayed translational movement, indicating that it detaches from the cell membrane. These observations suggest that dynamin-mediated scission involves a large torque generated by the coordinated actions of multiple dynamins in the helix, which is the main driving force for vesicle scission.