Open AccessThe protein kinase Akt acts as a coat adaptor in endocytic recycling
Author(s) -
Jong-Ping Hsu,
Ming Bai,
Kunhua Li,
JiaShu Yang,
Nam Chu,
Philip A. Cole,
Michael J. Eck,
Jian Li,
Victor Hsu
Publication year - 2020
Publication title -
nature cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 11.38
H-Index - 369
eISSN - 1476-4679
pISSN - 1465-7392
DOI - 10.1038/s41556-020-0530-z
Subject(s) - endocytic cycle , signal transducing adaptor protein , microbiology and biotechnology , protein kinase b , clathrin , phosphorylation , effector , clathrin adaptor proteins , biology , chemistry , endocytosis , biochemistry , receptor
Coat proteins have a central role in vesicular transport by binding to cargoes for their sorting into intracellular pathways. Cargo recognition is mediated by components of the coat complex known as adaptor proteins 1-3 . We previously showed that Arf-GAP with coil-coil, ANK repeat and PH domain-containing protein 1 (ACAP1) functions as an adaptor for a clathrin coat complex that has a function in endocytic recycling 4-6 . Here, we show that the protein kinase Akt acts as a co-adaptor in this complex, and is needed in conjunction with ACAP1 to bind to cargo proteins to promote their recycling. In addition to advancing the understanding of endocytic recycling, we uncover a fundamentally different function in which a kinase acts, as Akt in this case is an effector rather than a regulator in a cellular event.