Open AccessNuclear mechanosensing drives chromatin remodelling in persistently activated fibroblasts
Author(s) -
Cierra J. Walker,
Claudia Crocini,
Daniel Ramirez,
Anouk R. Killaars,
Joseph C. Grim,
Brian A. Aguado,
Kyle Clark,
Mary A. Allen,
Robin Dowell,
Leslie A. Leinwand,
Kristi S. Anseth
Publication year - 2021
Publication title -
nature biomedical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.961
H-Index - 56
ISSN - 2157-846X
DOI - 10.1038/s41551-021-00709-w
Subject(s) - myofibroblast , microbiology and biotechnology , chromatin , extracellular matrix , cytoskeleton , chemistry , lamin , histone , fibroblast , biology , fibrosis , pathology , in vitro , nucleus , biochemistry , dna , medicine , cell
Fibrotic disease is caused by the continuous deposition of extracellular matrix by persistently activated fibroblasts (also known as myofibroblasts), even after the resolution of the injury. Using fibroblasts from porcine aortic valves cultured on hydrogels that can be softened via exposure to ultraviolet light, here we show that increased extracellular stiffness activates the fibroblasts, and that cumulative tension on the nuclear membrane and increases in the activity of histone deacetylases transform transiently activated fibroblasts into myofibroblasts displaying condensed chromatin with genome-wide alterations. The condensed structure of the myofibroblasts is associated with cytoskeletal stability, as indicated by the inhibition of chromatin condensation and myofibroblast persistence after detachment of the nucleus from the cytoskeleton via the displacement of endogenous nesprins from the nuclear envelope. We also show that the chromatin structure of myofibroblasts from patients with aortic valve stenosis is more condensed than that of myofibroblasts from healthy donors. Our findings suggest that nuclear mechanosensing drives distinct chromatin signatures in persistently activated fibroblasts.