Open AccessPhytochrome B triggers light-dependent chromatin remodelling through the PRC2-associated PHD finger protein VIL1
Author(s) -
Jung-Hyun Kim,
Yogendra Bordiya,
Praveen Kumar Kathare,
Bo Zhao,
Wei Zong,
Enamul Huq,
Sibum Sung
Publication year - 2021
Publication title -
nature plants
Language(s) - English
Resource type - Journals
ISSN - 2055-0278
DOI - 10.1038/s41477-021-00986-y
Subject(s) - prc2 , chromatin , microbiology and biotechnology , chromatin remodeling , phytochrome , biology , biophysics , chemistry , genetics , ezh2 , dna , botany , red light
To compensate for a sessile nature, plants have developed sophisticated mechanisms to sense varying environmental conditions. Phytochromes (phys) are light and temperature sensors that regulate downstream genes to render plants responsive to environmental stimuli 1-4 . Here, we show that phyB directly triggers the formation of a repressive chromatin loop by physically interacting with VERNALIZATION INSENSITIVE 3-LIKE1/VERNALIZATION 5 (VIL1/VRN5), a component of Polycomb Repressive Complex 2 (PRC2) 5,6 , in a light-dependent manner. VIL1 and phyB cooperatively contribute to the repression of growth-promoting genes through the enrichment of Histone H3 Lys27 trimethylation (H3K27me3), a repressive histone modification. In addition, phyB and VIL1 mediate the formation of a chromatin loop to facilitate the repression of ATHB2. Our findings show that phyB directly utilizes chromatin remodelling to regulate the expression of target genes in a light-dependent manner.