Open AccessSystematic comparison of respiratory syncytial virus-induced memory B cell responses in two anatomical compartments
Author(s) -
Laila Shehata,
Wendy Wieland-Alter,
Daniel P. Maurer,
Eunice Chen,
Ruth I. Connor,
Peter F. Wright,
Laura M. Walker
Publication year - 2019
Publication title -
nature communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.559
H-Index - 365
ISSN - 2041-1723
DOI - 10.1038/s41467-019-09085-1
Subject(s) - memory b cell , antibody , b cell , immunology , virus , immunoglobulin d , biology , population , virology , respiratory system , adenoid , antibody response , medicine , environmental health , anatomy
Respiratory syncytial virus (RSV) is a leading cause of hospitalization in infants and young children. Although it is widely agreed that an RSV vaccine should induce both mucosal and systemic antibody responses, little is known about the B cell response to RSV in mucosa-associated lymphoid tissues. Here, we analyze this response by isolating 806 RSV F-specific antibodies from paired adenoid and peripheral blood samples from 4 young children. Overall, the adenoid-derived antibodies show higher binding affinities and neutralization potencies compared to antibodies isolated from peripheral blood. Approximately 25% of the neutralizing antibodies isolated from adenoids originate from a unique population of IgM + and/or IgD + memory B cells that contain a high load of somatic mutations but lack expression of classical memory B cell markers. Altogether, the results provide insight into the local B cell response to RSV and have implications for the development of vaccines that stimulate potent mucosal responses.