Open AccessTranscriptional regulation of endothelial cell behavior during sprouting angiogenesis
Author(s) -
HyunWoo Jeong,
Benjamín Hernández-Rodríguez,
JungMo Kim,
Kee-Pyo Kim,
Rocio Enriquez-Gasca,
Jong Ho Yoon,
Susanne Adams,
Hans R. Schöler,
Juan M. Vaquerizas,
Ralf H. Adams
Publication year - 2017
Publication title -
nature communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.559
H-Index - 365
ISSN - 2041-1723
DOI - 10.1038/s41467-017-00738-7
Subject(s) - angiogenesis , transcriptome , microbiology and biotechnology , biology , endothelial stem cell , transcription factor , regulator , in silico , sprouting angiogenesis , transcriptional regulation , regulation of gene expression , gene expression , gene , neovascularization , genetics , in vitro
Mediating the expansion of vascular beds in many physiological and pathological settings, angiogenesis requires dynamic changes in endothelial cell behavior. However, the molecular mechanisms governing endothelial cell activity during different phases of vascular growth, remodeling, maturation, and quiescence remain elusive. Here, we characterize dynamic gene expression changes during postnatal development and identify critical angiogenic factors in mouse retinal endothelial cells. Using actively translating transcriptome analysis and in silico computational analyses, we determine candidate regulators controlling endothelial cell behavior at different developmental stages. We further show that one of the identified candidates, the transcription factor MafB, controls endothelial sprouting in vitro and in vivo, and perform an integrative analysis of RNA-Seq and ChIP-Seq data to define putative direct MafB targets, which are activated or repressed by the transcriptional regulator. Together, our results identify novel cell-autonomous regulatory mechanisms controlling sprouting angiogenesis.