Open AccessA simple quantitative PCR assay to determine TRAMP transgene zygosity
Author(s) -
Ruidong Chen,
Xin Liang,
Mollianne M. Murray,
Ellen Karasik,
Jing Han,
Mingqing Zhu,
Barbara A. Foster,
Daniel E. Frigo,
Guocan Wang
Publication year - 2020
Publication title -
prostate cancer and prostatic diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2
H-Index - 64
eISSN - 1476-5608
pISSN - 1365-7852
DOI - 10.1038/s41391-020-00282-4
Subject(s) - tramp , prostate cancer , transgene , zygosity , genotype , biology , genotyping , genetically modified mouse , penetrance , cancer research , cancer , medicine , genetics , gene , phenotype
The TRansgenic Adenocarcinoma of the Mouse Prostate (TRAMP) model remains one of the most widely used transgenic mouse models of prostate cancer. This is due to its ability to recapitulate with ~100% penetrance multiple aspects of the human disease such as prostatic intraepithelial neoplasia lesions, invasive carcinoma, progression to castration-resistant prostate cancer including aggressive neuroendocrine prostate cancer and metastasis. Despite its popularity, the use of TRAMP mice is limited/slowed by the inability to distinguish the zygosity of the TRAMP transgene. This is especially true for breeding strategies implementing multiple crosses and alleles and when the rapid generation of large animal cohorts with the desired genotype is needed.