Open AccessPRMT5 is upregulated by B-cell receptor signaling and forms a positive-feedback loop with PI3K/AKT in lymphoma cells
Author(s) -
Fen Zhu,
Hui Guo,
Paul D. Bates,
Shanxiang Zhang,
Hui Zhang,
Krystle Nomie,
Yangguang Li,
Li Lü,
Kaitlyn R. Seibold,
Fangyu Wang,
Ian Rumball,
Hunter Cameron,
Nguyet M. Hoang,
David T. Yang,
Wei Xu,
Liang Zhang,
Michael Wang,
Christian M. Capitini,
Rui Li
Publication year - 2019
Publication title -
leukemia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.539
H-Index - 192
eISSN - 1476-5551
pISSN - 0887-6924
DOI - 10.1038/s41375-019-0489-6
Subject(s) - protein kinase b , protein arginine methyltransferase 5 , pi3k/akt/mtor pathway , cancer research , germinal center , biology , cell growth , signal transduction , diffuse large b cell lymphoma , b cell , lymphoma , breakpoint cluster region , microbiology and biotechnology , immunology , receptor , methyltransferase , genetics , gene , methylation , antibody
PRMT5, which regulates gene expression by symmetric dimethylation of histones and non-histone target proteins, is overexpressed and plays a pathogenic role in many cancers. In diffuse large B cell lymphoma (DLBCL), the mechanisms of PRMT5 dysregulation and its role in lymphomagenesis remain largely unknown. Here we demonstrate that B cell receptor (BCR) signaling regulates PRMT5 expression in DLBCL cells. Immunohistochemical analysis reveals elevated levels of PRMT5 expression in DLBCL cases and in germinal center (GC) B cells when compared to naive B cells. PRMT5 can be induced in naive B cells by BCR stimulation. We discovered that BTK-NF-κB signaling induces PRMT5 transcription in activated B cell-like (ABC) DLBCL cells while BCR downstream PI3K-AKT-MYC signaling upregulates PRMT5 expression in both ABC and GCB DLBCL cells. PRMT5 inhibition inhibits the growth of DLBCL cells in vitro and patient derived xenografts. Genomic and biochemical analysis demonstrate that PRMT5 promotes cell cycle progression and activates PI3K-AKT signaling, suggesting a feedback regulatory mechanism to enhance cell survival and proliferation. Co-targeting PRMT5 and AKT by their specific inhibitors is lethal to DLBCL cell lines and primary cancer cells. Therefore, this study provides a mechanistic rationale for clinical trials to evaluate PRMT5 and AKT inhibitors for DLBCL.