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PPARγ Expression After a High‐fat Meal Is Associated With Plasma Superoxide Dismutase Activity in Morbidly Obese Persons
Author(s) -
GarciaFuentes Eduardo,
Murri Mora,
GarridoSanchez Lourdes,
GarciaSerrano Sara,
GarcíaAlmeida Jose M.,
MorenoSantos Inmaculada,
Tinahones Francisco J.,
MaciasGonzalez Manuel
Publication year - 2010
Publication title -
obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.438
H-Index - 199
eISSN - 1930-739X
pISSN - 1930-7381
DOI - 10.1038/oby.2009.314
Subject(s) - medicine , endocrinology , superoxide dismutase , peripheral blood mononuclear cell , peroxisome proliferator activated receptor , dismutase , chemistry , antioxidant , meal , glutathione , insulin resistance , receptor , insulin , enzyme , oxidative stress , biochemistry , in vitro
Peroxisome proliferator‐activated receptor‐γ (PPARγ) may play a protective role in the regulation of vascular function, partly mediated by its effects on superoxide dismutase (SOD). The aim of this study was to determine the association between PPARγ expression in peripheral blood mononuclear cells (PBMCs) and SOD activity in morbidly obese persons with varying degrees of insulin resistance (IR). We studied in 10 morbidly obese persons (five with no IR and five with high IR) the effect of a high‐fat meal on the plasma activity of various antioxidant enzymes and the mRNA expression of PPARγ in PBMC. The high‐fat meal resulted in a significant decrease in plasma SOD activity, glutathione reductase (GSH‐Rd) activity, and mRNA expression of PPARγ only in the group of morbidly obese persons with high IR. PPARγ expression after the high‐fat meal correlated with the IR levels ( r = −0.803, P = 0.009) and the plasma SOD activity ( r = 0.903, P = 0.001). Likewise, the reduction in PPARγ expression correlated with the increase in free fatty acids (FFA) ( r = 0.733, P = 0.016). In conclusion, the decreased expression of PPARγ in PBMC in morbidly obese persons after a high‐fat meal was associated with the state of IR, the plasma SOD activity, and the changes in the concentration of FFA.