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LMNA mRNA Expression Is Altered in Human Obesity and Type 2 Diabetes
Author(s) -
Miranda Merce,
Chacón Matilde R.,
Gutiérrez Cristina,
Vilarrasa Nuria,
Gómez Jose M.,
Caubet Enric,
Megía Ana,
Vendrell Joan
Publication year - 2008
Publication title -
obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.438
H-Index - 199
eISSN - 1930-739X
pISSN - 1930-7381
DOI - 10.1038/oby.2008.276
Subject(s) - lmna , type 2 diabetes , lamin , medicine , endocrinology , adipose tissue , glycemic , obesity , diabetes mellitus , messenger rna , adipocyte , adipogenesis , biology , gene , genetics
Objective: The aim of this study was to analyze lamin A/C mRNA levels in abdominal subcutaneous adipose tissue in two conditions—obesity and type 2 diabetes—that share common inflammatory and metabolic features, and to assess their relationship with selected inflammatory and adipogenic genes. Methods and Procedures: This is a cross‐sectional study involving 52 nondiabetic and 54 type 2 diabetes patients. Anthropometrical and analytical measurements (glycemic, lipidic, and inflammatory profiles) were performed, and mRNA expression was determined using real‐time PCR. Results: Lamin A and C isoforms are expressed differentially. Lamin A/C mRNA levels were increased in obese and in type 2 diabetes patients. We also observed a strong relationship between both isoforms ( B = 2.218, P < 0.001) and among lamin C mRNA expression and adipogenic (sterol‐responsive element binding protein‐1 ( SREBP1c )) and inflammatory (interleukin‐6 ( IL‐6 )) markers ( B = 0.854, P = 0.001, and B = 0.557, P < 0.001, respectively). Discussion: These data suggest that lamin A/C may be involved in the adipocyte gene profile observed in obesity and type 2 diabetes.