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Effects of Pronounced Weight Loss on Adiponectin Oligomer Composition and Metabolic Parameters
Author(s) -
Engl Julia,
Bobbert Thomas,
Ciardi Christian,
Laimer Markus,
Tatarczyk Tobias,
Kaser Susanne,
Weiss Helmut,
Molnar Clemens,
Tilg Herbert,
Patsch Josef R.,
Spranger Joachim,
Ebenbichler Christoph F.
Publication year - 2007
Publication title -
obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.438
H-Index - 199
eISSN - 1930-739X
pISSN - 1930-7381
DOI - 10.1038/oby.2007.627
Subject(s) - adiponectin , weight loss , medicine , endocrinology , anthropometry , chemistry , western blot , gene isoform , adipose tissue , obesity , insulin resistance , biochemistry , gene
Objective: Adiponectin is an adipocytokine secreted into circulation in three isoforms. The aim of the study was to investigate changes of adiponectin isoforms during profound weight loss and its relation to anthropomorphometric and metabolic parameters. Research Methods and Procedures: Thirteen severely obese female subjects were examined before and 1 year after surgical treatment. Total adiponectin was determined by radioimmunosorbent assay, and oligomer composition was detected by nondenaturing Western blot. Results: BMI decreased substantially ( p < 0.001), which was associated with an increase of total adiponectin from 12.9 ± 5.9 to 14.3 ± 6.1 μg/mL ( p = 0.055). Medium molecular weight (MMW) adiponectin increased from 7.5 ± 3.6 to 9.1 ± 4.1 μg/mL ( p = 0.009), whereas high (HMW) and low molecular weight adiponectin remained unchanged. Δ values of total adiponectin correlated significantly with Δ values of anthropometric parameters. Similar correlations were found for Δ values of MMW (Δ weight: r 2 = 0.4132, p = 0.0178; Δ BMI: r 2 = 0.3319, p = 0.0393; Δ fat mass: r 2 = 0.5202, p = 0.0054). Discussion: Thus, profound weight loss was associated with an increase in total adiponectin, which was mainly and consistently caused by increases in MMW adiponectin ( p = 0.009). These changes result in a shift from low molecular weight to MMW and HMW adiponectin isoforms, which may be related to improvements in both anthropometric and metabolic parameters.