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Macrophage‐Colony Stimulating Factor in Obese Adipose Tissue: Studies With Heterozygous op /+ Mice
Author(s) -
Sugita Satoshi,
Kamei Yasutomi,
Oka JunIchiro,
Suganami Takayoshi,
Ogawa Yoshihiro
Publication year - 2007
Publication title -
obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.438
H-Index - 199
eISSN - 1930-739X
pISSN - 1930-7381
DOI - 10.1038/oby.2007.237
Subject(s) - adipose tissue , medicine , endocrinology , cd68 , adipose tissue macrophages , monocyte , macrophage , gene expression , macrophage colony stimulating factor , obesity , biology , inflammation , gene , white adipose tissue , immunohistochemistry , in vitro , biochemistry
Objective: We examined the gene expression of macrophage‐colony stimulating factor (M‐CSF) in mice with diet‐induced obesity and in genetically obese mice. We also examined the effect of decreased M‐CSF signaling on the susceptibility to obesity and macrophage recruitment into the adipose tissue of mice. Research Methods and Procedures: The adipose tissue from mice with diet‐induced obesity, obese KK A y mice, and ob/ob obese mice was used for RNA preparation. Production of M‐CSF and monocyte chemoattractant protein‐1 (MCP‐1) was examined by quantitative real‐time polymerase chain reaction (PCR) and enzyme‐linked immunosorbent assay. The op /+ heterozygous mice, with decreased functional M‐CSF expression, were placed on a high‐fat diet or crossed with KK A y mice to study the susceptibility to obesity. The gene expression of macrophage markers in adipose tissue was examined. Results: The expression of M‐CSF was not significantly changed in mice on a high‐fat diet or in either type of genetic obesity (KK A y or ob/ob mice). No change in the degree of obesity or macrophage‐related gene expression (F4/80, CD68, and MCP‐1) in the adipose tissue was observed in op /+ mice compared with +/+ control mice, which were either treated with a high‐fat diet or crossed with KK A y mice. Discussion: This study demonstrated that there was no significant change in the expression of M‐CSF in the adipose tissue from obese mice and only a minor phenotypic change, such as macrophage infiltration, in the adipose tissue from op /+ mice, suggesting that M‐CSF does not play a major role in macrophage recruitment in the adipose tissue of obese mice.