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Effect of Exercise on Hepatic Gene Expression in an Obese Mouse Model Using cDNA Microarrays
Author(s) -
Lee KyoungYoung,
Kim SuJong,
Cha YounSoo,
So JuRyun,
Park JoonSuk,
Kang KyungSun,
Chon TaeWon
Publication year - 2006
Publication title -
obesity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.438
H-Index - 199
eISSN - 1930-739X
pISSN - 1930-7381
DOI - 10.1038/oby.2006.147
Subject(s) - medicine , endocrinology , steatosis , gene expression , microarray , lipid metabolism , obesity , complementary dna , gene , biology , biochemistry
To understand the molecular mechanisms involved in the effect of exercise training, we examined hepatic transcriptional profiles using cDNA microarrays in exercise‐trained and untrained mice with diet‐induced obesity. C57BL/6J male mice ( n = 10/group) were fed with a normal diet, high‐fat diet (HFD), or HFD with exercise training for 12 weeks. The expression level of ∼10,000 transcripts in liver tissues from each group was assessed using cDNA microarray analysis. Exercise training improved lipid profiles and hepatic steatosis and decreased body fat mass induced by the HFD. Seventy‐three genes were differentially expressed in the HFD‐ and/or HFD with exercise training‐treated groups, compared with the normal diet‐ and HFD‐fed groups, respectively. Interestingly, the expression profiles involved in metabolism, such as elongation of very long chain fatty acids‐like 2, lipin, and malic enzyme, were changed by exercise training. In addition, expression of genes altered by exercise training related to defense and stress response, including metallothionein 1 and 2 and heat shock protein, showed interesting findings. Our study showed beneficial effects of exercise training in preventing the development of obesity and metabolic disorders in mice with diet‐induced obesity.