Open AccessImaging of anticancer drug action in single cells
Author(s) -
Miles A. Miller,
Ralph Weissleder
Publication year - 2017
Publication title -
nature reviews. cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 19.575
H-Index - 442
eISSN - 1474-1768
pISSN - 1474-175X
DOI - 10.1038/nrc.2017.41
Subject(s) - drug action , drug , medicine , cell , drug delivery , in vivo , cancer cell , cancer research , pharmacology , cancer , biology , nanotechnology , materials science , microbiology and biotechnology , genetics
Imaging is widely used in anticancer drug development, typically for whole-body tracking of labelled drugs to different organs or to assess drug efficacy through volumetric measurements. However, increasing attention has been drawn to pharmacology at the single-cell level. Diverse cell types, including cancer-associated immune cells, physicochemical features of the tumour microenvironment and heterogeneous cell behaviour all affect drug delivery, response and resistance. This Review summarizes developments in the imaging of in vivo anticancer drug action, with a focus on microscopy approaches at the single-cell level and translational lessons for the clinic.