Applying of novel subtracted method Genetically Directed Differential Subtraction Chain (GDDSC) in plant genomes | Zendy

Ewa Siedlecka | Zendy; Aneta HromadaJudycka | Zendy; Magdalena Pawełkowicz | Zendy; Rafał Wóycicki | Zendy; Monika RakoczyTrojanowska | Zendy; Zbigniew Przybecki | Zendy

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Applying of novel subtracted method Genetically Directed Differential Subtraction Chain (GDDSC) in plant genomes

Author(s) -

Ewa Siedlecka,

Aneta HromadaJudycka,

Magdalena Pawełkowicz,

Rafał Wóycicki,

Monika RakoczyTrojanowska,

Zbigniew Przybecki

Publication year - 2010

Publication title -

nature precedings

Language(s) - English

Resource type - Journals

ISSN - 1756-0357

DOI - 10.1038/npre.2010.5465.1

Subject(s) - secale , biology , genome , cucumis , subtraction , dna sequencing , gene , computational biology , genetics , botany , mathematics , arithmetic

We present a simple subtraction procedure of GDDSC as a modification of the original DSC and GDRDA methods. Genetically Directed Differential Subtraction Chain (GDDSC) is a process by which highly related genomes are compared in order to isolate tags carrying the polymorphisms. To detect specific DNA fragment (tag), we can then monitor offspring plants for efficient molecular breeding. The GDDSC protocol was applied to isolation of new sex related clones from cucumber plants (_Cucumis_ _sativus_ L.) and new tissue culture response clones from rye (_Secale_ _cereale_ L.). The newly identified tags, obtained by GDDSC represent pools of candidate genes and other sequences, which could serve as potential markers for requested traits

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