Isolation and in vitro expansion of human colonic stem cells | Zendy

Peter Jung | Zendy; Toshiro Sato | Zendy; Anna MerlosSuárez | Zendy; Francisco M. Barriga | Zendy; Mar Iglesias | Zendy; David Rossell | Zendy; Herbert Auer | Zendy; Mercedes Gallardo | Zendy; Marı́a A. Blasco | Zendy; Elena Sancho | Zendy; Hans Clevers | Zendy; Eduard Batlle | Zendy

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Isolation and in vitro expansion of human colonic stem cells

Author(s) -

Peter Jung,

Toshiro Sato,

Anna MerlosSuárez,

Francisco M. Barriga,

Mar Iglesias,

David Rossell,

Herbert Auer,

Mercedes Gallardo,

Marı́a A. Blasco,

Elena Sancho,

Hans Clevers,

Eduard Batlle

Publication year - 2011

Publication title -

nature medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 19.536

H-Index - 547

eISSN - 1546-170X

pISSN - 1078-8956

DOI - 10.1038/nm.2470

Subject(s) - stem cell , biology , microbiology and biotechnology , in vitro , population , epithelium , cell type , cell , stem cell niche , intestinal epithelium , progenitor cell , genetics , medicine , environmental health

Here we describe the isolation of stem cells of the human colonic epithelium. Differential cell surface abundance of ephrin type-B receptor 2 (EPHB2) allows the purification of different cell types from human colon mucosa biopsies. The highest EPHB2 surface levels correspond to epithelial colonic cells with the longest telomeres and elevated expression of intestinal stem cell (ISC) marker genes. Moreover, using culturing conditions that recreate the ISC niche, a substantial proportion of EPHB2-high cells can be expanded in vitro as an undifferentiated and multipotent population.

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