Open AccessIntegrated proteomic and transcriptomic profiling of mouse lung development and Nmyc target genes
Author(s) -
Cox Brian,
Kislinger Thomas,
Wigle Dennis A,
Kannan Anitha,
Brown Kevin,
Okubo Tadashi,
Hogan Brigid,
Jurisica Igor,
Frey Brendan,
Rossant Janet,
Emili Andrew
Publication year - 2007
Publication title -
molecular systems biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 8.523
H-Index - 148
ISSN - 1744-4292
DOI - 10.1038/msb4100151
Subject(s) - biology , transcriptome , gene , gene expression profiling , gene expression , microarray analysis techniques , microarray , messenger rna , dna microarray , microbiology and biotechnology , genetics
Although microarray analysis has provided information regarding the dynamics of gene expression during development of the mouse lung, no extensive correlations have been made to the levels of corresponding protein products. Here, we present a global survey of protein expression during mouse lung organogenesis from embryonic day E13.5 until adulthood using gel‐free two‐dimensional liquid chromatography coupled to shotgun tandem mass spectrometry (MudPIT). Mathematical modeling of the proteomic profiles with parallel DNA microarray data identified large groups of gene products with statistically significant correlation or divergence in coregulation of protein and transcript levels during lung development. We also present an integrative analysis of mRNA and protein expression in Nmyc loss‐ and gain‐of‐function mutants. This revealed a set of 90 positively and negatively regulated putative target genes. These targets are evidence that Nmyc is a regulator of genes involved in mRNA processing and a repressor of the imprinted gene Igf2r in the developing lung.