Open AccessCore transcriptional regulatory circuitry in human hepatocytes
Author(s) -
Odom Duncan T,
Dowell Robin D,
Jacobsen Elizabeth S,
Nekludova Lena,
Rolfe P Alexander,
Danford Timothy W,
Gifford David K,
Fraenkel Ernest,
Bell Graeme I,
Young Richard A
Publication year - 2006
Publication title -
molecular systems biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 8.523
H-Index - 148
ISSN - 1744-4292
DOI - 10.1038/msb4100059
Subject(s) - biology , computational biology , core (optical fiber) , gene regulatory network , microbiology and biotechnology , regulation of gene expression , transcriptional regulation , transcription factor , genetics , gene , gene expression , materials science , composite material
We mapped the transcriptional regulatory circuitry for six master regulators in human hepatocytes using chromatin immunoprecipitation and high‐resolution promoter microarrays. The results show that these regulators form a highly interconnected core circuitry, and reveal the local regulatory network motifs created by regulator–gene interactions. Autoregulation was a prominent theme among these regulators. We found that hepatocyte master regulators tend to bind promoter regions combinatorially and that the number of transcription factors bound to a promoter corresponds with observed gene expression. Our studies reveal portions of the core circuitry of human hepatocytes.