Open AccessSequential induction of auxin efflux and influx carriers regulates lateral root emergence
Author(s) -
Péret Benjamin,
Middleton Alistair M,
French Andrew P,
Larrieu Antoine,
Bishopp Anthony,
Njo Maria,
Wells Darren M,
Porco Silvana,
Mellor Nathan,
Band Leah R,
Casimiro Ilda,
KleineVehn Jürgen,
Vanneste Steffen,
Sairanen Ilkka,
Mallet Romain,
Sandberg Göran,
Ljung Karin,
Beeckman Tom,
Benkova Eva,
Friml Jiří,
Kramer Eric,
King John R,
De Smet Ive,
Pridmore Tony,
Owen Markus,
Bennett Malcolm J
Publication year - 2013
Publication title -
molecular systems biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 8.523
H-Index - 148
ISSN - 1744-4292
DOI - 10.1038/msb.2013.43
Subject(s) - pericycle , auxin , primordium , biology , lateral root , microbiology and biotechnology , arabidopsis , efflux , cell , biophysics , biochemistry , gene , mutant
In Arabidopsis , lateral roots originate from pericycle cells deep within the primary root. New lateral root primordia (LRP) have to emerge through several overlaying tissues. Here, we report that auxin produced in new LRP is transported towards the outer tissues where it triggers cell separation by inducing both the auxin influx carrier LAX3 and cell‐wall enzymes. LAX3 is expressed in just two cell files overlaying new LRP. To understand how this striking pattern of LAX3 expression is regulated, we developed a mathematical model that captures the network regulating its expression and auxin transport within realistic three‐dimensional cell and tissue geometries. Our model revealed that, for the LAX3 spatial expression to be robust to natural variations in root tissue geometry, an efflux carrier is required—later identified to be PIN3. To prevent LAX3 from being transiently expressed in multiple cell files, PIN3 and LAX3 must be induced consecutively, which we later demonstrated to be the case. Our study exemplifies how mathematical models can be used to direct experiments to elucidate complex developmental processes.