Open AccessA global S. cerevisiae small ubiquitin‐related modifier (SUMO) system interactome
Author(s) -
Srikumar Tharan,
Lewicki Megan C,
Raught Brian
Publication year - 2013
Publication title -
molecular systems biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 8.523
H-Index - 148
ISSN - 1744-4292
DOI - 10.1038/msb.2013.23
Subject(s) - interactome , biology , nucleoporin , computational biology , saccharomyces cerevisiae , budding yeast , ubiquitin , tandem affinity purification , ribosomal protein , sumo protein , proteases , ubiquitin protein ligases , protein–protein interaction , proteomics , yeast , biochemistry , ubiquitin ligase , ribosome , affinity chromatography , nuclear protein , enzyme , rna , transcription factor , gene
The small ubiquitin‐related modifier (SUMO) system has been implicated in a number of biological functions, yet the individual components of the SUMO machinery involved in each of these activities were largely unknown. Here we report the first global SUMO system interactome. Using affinity purification coupled with mass spectrometry, we identify >450 protein–protein interactions surrounding the SUMO E2, Siz type E3s and SUMO‐specific proteases in budding yeast. Exploiting this information‐rich resource, we validate several Siz1‐ and Siz2‐specific substrates, identify a nucleoporin required for proper Ulp1 localization, and uncover important new roles for Ubc9 and Ulp2 in the maintenance of ribosomal DNA.