Open AccessThe quantitative proteome of a human cell line
Author(s) -
Beck Martin,
Schmidt Alexander,
Malmstroem Johan,
Claassen Manfred,
Ori Alessandro,
Szymborska Anna,
Herzog Franz,
Rinner Oliver,
Ellenberg Jan,
Aebersold Ruedi
Publication year - 2011
Publication title -
molecular systems biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 8.523
H-Index - 148
ISSN - 1744-4292
DOI - 10.1038/msb.2011.82
Subject(s) - proteome , biology , interphase , computational biology , systems biology , mitosis , function (biology) , proteomics , human proteome project , quantitative proteomics , human cell , microbiology and biotechnology , bioinformatics , cell culture , genetics , gene
The generation of mathematical models of biological processes, the simulation of these processes under different conditions, and the comparison and integration of multiple data sets are explicit goals of systems biology that require the knowledge of the absolute quantity of the system's components. To date, systematic estimates of cellular protein concentrations have been exceptionally scarce. Here, we provide a quantitative description of the proteome of a commonly used human cell line in two functional states, interphase and mitosis. We show that these human cultured cells express at least ∼10 000 proteins and that the quantified proteins span a concentration range of seven orders of magnitude up to 20 000 000 copies per cell. We discuss how protein abundance is linked to function and evolution.