Open AccessA novel strategy for the comprehensive analysis of the biomolecular composition of isolated plasma membranes
Author(s) -
Thimiri Govinda Raj Deepak B,
Ghesquière Bart,
Tharkeshwar Arun Kumar,
Coen Katrijn,
Derua Rita,
Vanderschaeghe Dieter,
Rysman Evelien,
Bagadi Murali,
Baatsen Pieter,
De Strooper Bart,
Waelkens Etienne,
Borghs Gustaaf,
Callewaert Nico,
Swinnen Johan,
Gevaert Kris,
Annaert Wim
Publication year - 2011
Publication title -
molecular systems biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 8.523
H-Index - 148
ISSN - 1744-4292
DOI - 10.1038/msb.2011.74
Subject(s) - biology , computational biology , membrane , composition (language) , biochemistry , philosophy , linguistics
We manufactured a novel type of lipid‐coated superparamagnetic nanoparticles that allow for a rapid isolation of plasma membranes (PMs), enabling high‐resolution proteomic, glycomic and lipidomic analyses of the cell surface. We used this technology to characterize the effects of presenilin knockout on the PM composition of mouse embryonic fibroblasts. We found that many proteins are selectively downregulated at the cell surface of presenilin knockout cells concomitant with lowered surface levels of cholesterol and certain sphingomyelin species, indicating defects in specific endosomal transport routes to and/or from the cell surface. Snapshots of N ‐glycoproteomics and cell surface glycan profiling further underscored the power and versatility of this novel methodology. Since PM proteins provide many pathologically relevant biomarkers representing two‐thirds of the currently used drug targets, this novel technology has great potential for biomedical and pharmaceutical applications.