Open AccessToward an understanding of the protein interaction network of the human liver
Author(s) -
Wang Jian,
Huo Keke,
Ma Lixin,
Tang Liujun,
Li Dong,
Huang Xiaobi,
Yuan Yanzhi,
Li Chunhua,
Wang Wei,
Guan Wei,
Chen Hui,
Jin Chaozhi,
Wei Junchen,
Zhang Wanqiao,
Yang Yongsheng,
Liu Qiongming,
Zhou Ying,
Zhang Cuili,
Wu Zhihao,
Xu Wangxiang,
Zhang Ying,
Liu Tao,
Yu Donghui,
Zhang Yaping,
Chen Liang,
Zhu Dewu,
Zhong Xing,
Kang Lixin,
Gan Xiang,
Yu Xiaolan,
Ma Qi,
Yan Jing,
Zhou Li,
Liu Zhongyang,
Zhu Yunping,
Zhou Tao,
He Fuchu,
Yang Xiaoming
Publication year - 2011
Publication title -
molecular systems biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 8.523
H-Index - 148
ISSN - 1744-4292
DOI - 10.1038/msb.2011.67
Subject(s) - biology , proteome , human liver , protein–protein interaction , computational biology , interaction network , phenotype , systems biology , protein interaction networks , yeast , drug discovery , liver disease , bioinformatics , genetics , biochemistry , enzyme , gene
Proteome‐scale protein interaction maps are available for many organisms, ranging from bacteria, yeast, worms and flies to humans. These maps provide substantial new insights into systems biology, disease research and drug discovery. However, only a small fraction of the total number of human protein–protein interactions has been identified. In this study, we map the interactions of an unbiased selection of 5026 human liver expression proteins by yeast two‐hybrid technology and establish a human liver protein interaction network (HLPN) composed of 3484 interactions among 2582 proteins. The data set has a validation rate of over 72% as determined by three independent biochemical or cellular assays. The network includes metabolic enzymes and liver‐specific, liver‐phenotype and liver‐disease proteins that are individually critical for the maintenance of liver functions. The liver enriched proteins had significantly different topological properties and increased our understanding of the functional relationships among proteins in a liver‐specific manner. Our data represent the first comprehensive description of a HLPN, which could be a valuable tool for understanding the functioning of the protein interaction network of the human liver.