Open Access
Arabidopsis G‐protein interactome reveals connections to cell wall carbohydrates and morphogenesis
Author(s) -
Klopffleisch Karsten,
Phan Nguyen,
Augustin Kelsey,
Bayne Robert S,
Booker Katherine S,
Botella Jose R,
Carpita Nicholas C,
Carr Tyrell,
Chen JinGui,
Cooke Thomas Ryan,
FrickCheng Arwen,
Friedman Erin J,
Fulk Brandon,
Hahn Michael G,
Jiang Kun,
Jorda Lucia,
Kruppe Lydia,
Liu Chenggang,
Lorek Justine,
McCann Maureen C,
Molina Antonio,
Moriyama Etsuko N,
Mukhtar M Shahid,
Mudgil Yashwanti,
Pattathil Sivakumar,
Schwarz John,
Seta Steven,
Tan Matthew,
Temp Ulrike,
Trusov Yuri,
Urano Daisuke,
Welter Bastian,
Yang Jing,
Panstruga Ralph,
Uhrig Joachim F,
Jones Alan M
Publication year - 2011
Publication title -
molecular systems biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 8.523
H-Index - 148
ISSN - 1744-4292
DOI - 10.1038/msb.2011.66
Subject(s) - interactome , heterotrimeric g protein , biology , g protein coupled receptor , effector , bimolecular fluorescence complementation , protein–protein interaction , g protein , microbiology and biotechnology , protein subunit , proteomics , computational biology , signal transduction , gene , genetics
The heterotrimeric G‐protein complex is minimally composed of Gα, Gβ, and Gγ subunits. In the classic scenario, the G‐protein complex is the nexus in signaling from the plasma membrane, where the heterotrimeric G‐protein associates with heptahelical G‐protein‐coupled receptors (GPCRs), to cytoplasmic target proteins called effectors. Although a number of effectors are known in metazoans and fungi, none of these are predicted to exist in their canonical forms in plants. To identify ab initio plant G‐protein effectors and scaffold proteins, we screened a set of proteins from the G‐protein complex using two‐hybrid complementation in yeast. After deep and exhaustive interrogation, we detected 544 interactions between 434 proteins, of which 68 highly interconnected proteins form the core G‐protein interactome. Within this core, over half of the interactions comprising two‐thirds of the nodes were retested and validated as genuine in planta . Co‐expression analysis in combination with phenotyping of loss‐of‐function mutations in a set of core interactome genes revealed a novel role for G‐proteins in regulating cell wall modification.