Open AccessMolecular characterization of the evolution of phagosomes
Author(s) -
Boulais Jonathan,
Trost Matthias,
Landry Christian R,
Dieckmann Régis,
Levy Emmanuel D,
Soldati Thierry,
Michnick Stephen W,
Thibault Pierre,
Desjardins Michel
Publication year - 2010
Publication title -
molecular systems biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 8.523
H-Index - 148
ISSN - 1744-4292
DOI - 10.1038/msb.2010.80
Subject(s) - phagosome , biology , proteome , organelle , acquired immune system , innate immune system , phagocytosis , microbiology and biotechnology , immune system , multicellular organism , computational biology , gene , immunology , bioinformatics , genetics
Amoeba use phagocytosis to internalize bacteria as a source of nutrients, whereas multicellular organisms utilize this process as a defense mechanism to kill microbes and, in vertebrates, initiate a sustained immune response. By using a large‐scale approach to identify and compare the proteome and phosphoproteome of phagosomes isolated from distant organisms, and by comparative analysis over 39 taxa, we identified an ‘ancient’ core of phagosomal proteins around which the immune functions of this organelle have likely organized. Our data indicate that a larger proportion of the phagosome proteome, compared with the whole cell proteome, has been acquired through gene duplication at a period coinciding with the emergence of innate and adaptive immunity. Our study also characterizes in detail the acquisition of novel proteins and the significant remodeling of the phagosome phosphoproteome that contributed to modify the core constituents of this organelle in evolution. Our work thus provides the first thorough analysis of the changes that enabled the transformation of the phagosome from a phagotrophic compartment into an organelle fully competent for antigen presentation.