Open AccessHepatitis C virus infection protein network
Author(s) -
de Chassey B,
Navratil V,
Tafforeau L,
Hiet M S,
AublinGex A,
Agaugué S,
Meiffren G,
Pradezynski F,
Faria B F,
Chantier T,
Le Breton M,
Pellet J,
Davoust N,
Mangeot P E,
Chaboud A,
Penin F,
Jacob Y,
Vidalain P O,
Vidal M,
André P,
RabourdinCombe C,
Lotteau V
Publication year - 2008
Publication title -
molecular systems biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 8.523
H-Index - 148
ISSN - 1744-4292
DOI - 10.1038/msb.2008.66
Subject(s) - interactome , biology , ns5a , proteome , hepatitis c virus , ns3 , computational biology , hepacivirus , proteomics , protein–protein interaction , interaction network , virology , virus , bioinformatics , microbiology and biotechnology , genetics , gene
A proteome‐wide mapping of interactions between hepatitis C virus (HCV) and human proteins was performed to provide a comprehensive view of the cellular infection. A total of 314 protein–protein interactions between HCV and human proteins was identified by yeast two‐hybrid and 170 by literature mining. Integration of this data set into a reconstructed human interactome showed that cellular proteins interacting with HCV are enriched in highly central and interconnected proteins. A global analysis on the basis of functional annotation highlighted the enrichment of cellular pathways targeted by HCV. A network of proteins associated with frequent clinical disorders of chronically infected patients was constructed by connecting the insulin, Jak/STAT and TGFβ pathways with cellular proteins targeted by HCV. CORE protein appeared as a major perturbator of this network. Focal adhesion was identified as a new function affected by HCV, mainly by NS3 and NS5A proteins.