Open AccessInduction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions
Author(s) -
Sang Choel Lee,
Seung Hyeok Han,
Jin Ji Li,
Sun Ha Lee,
Dong-Sub Jung,
Seung-Jae Kwak,
Seung Hye Kim,
Dong Ki Kim,
TaeHyun Yoo,
Jin Hyun Kim,
Se-Ho Chang,
Dae Suk Han,
ShinWook Kang
Publication year - 2009
Publication title -
kidney international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.499
H-Index - 276
eISSN - 1523-1755
pISSN - 0085-2538
DOI - 10.1038/ki.2009.286
Subject(s) - podocyte , heme oxygenase , apoptosis , downregulation and upregulation , endocrinology , medicine , streptozotocin , chemistry , podocalyxin , heme , in vivo , biology , microbiology and biotechnology , kidney , diabetes mellitus , biochemistry , enzyme , proteinuria , gene
Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose-treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose-treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.
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