Differential distribution of the Sodium-vitamin C cotransporter-1 along the proximal tubule of the mouse and human kidney | Zendy

Tamara Castro | Zendy; Marcela Low | Zendy; Katterine Salazar | Zendy; Hernán Montecinos | Zendy; Manuel Cifuentes | Zendy; Alejandro J. Yáñez | Zendy; Juan C. Slebe | Zendy; Carlos D. Figueroa | Zendy; Karin Reinicke | Zendy; María de los Ángeles García | Zendy; Juan Pablo Henríquez | Zendy; Francisco Nualart | Zendy

Open Access

Differential distribution of the Sodium-vitamin C cotransporter-1 along the proximal tubule of the mouse and human kidney

Author(s) -

Tamara Castro,

Marcela Low,

Katterine Salazar,

Hernán Montecinos,

Manuel Cifuentes,

Alejandro J. Yáñez,

Juan C. Slebe,

Carlos D. Figueroa,

Karin Reinicke,

María de los Ángeles García,

Juan Pablo Henríquez,

Francisco Nualart

Publication year - 2008

Publication title -

kidney international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.499

H-Index - 276

eISSN - 1523-1755

pISSN - 0085-2538

DOI - 10.1038/ki.2008.329

Subject(s) - cotransporter , proximal tubule , kidney tubules , kidney , endocrinology , human kidney , sodium , medicine , renal tubule , distribution (mathematics) , distal convoluted tubule , chemistry , biology , nephron , organic chemistry , mathematical analysis , mathematics

Vitamin C is reabsorbed from the renal lumen by one isoform of sodium-vitamin C co-transporters that mediate high affinity sodium-dependent L-ascorbic acid transport. Sodium-vitamin C cotransporter-1 mRNA has been detected in intestine and liver and the S3 segment of the renal proximal tubule. Here, we found that its distribution was broader and all three proximal tubule segments of mouse and human expressed the transporter but the S3 segment had the highest expression. Sodium-vitamin C co-transporter-1 expression was also found in the renal epithelial-derived LLC-PK1 cell line. Ascorbic acid transport in these cells was regulated by a single kinetic component that depended on the sodium concentration, pH and temperature. Reducing ascorbate concentration increased the apical expression of the transporter suggesting the presence of a feedback system for regulation of transporter abundance at the luminal membrane.

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