Premium
The reinvention of twentieth century microscopy for three‐dimensional imaging
Author(s) -
Whitehead Lachlan W,
McArthur Kate,
Geoghegan Niall D,
Rogers Kelly L
Publication year - 2017
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.2017.36
Subject(s) - light sheet fluorescence microscopy , microscopy , optical sectioning , confocal microscopy , nanotechnology , confocal , fluorescence microscope , microscope , hyperspectral imaging , imaging technology , computer science , optics , materials science , artificial intelligence , fluorescence , physics , scanning confocal electron microscopy , geology , remote sensing
In just over a decade, the field of biomedical research has witnessed a radical evolution in technologies for the 3‐ and 4‐dimensional imaging of biological samples. Light sheet fluorescence microscopy is quickly developing into a powerful approach for fast, volumetric imaging of cells, tissues and living organisms. This review touches on the development of 3‐dimensional imaging, from its foundations, namely from the invention of confocal microscopy in the twentieth century to more recent examples, notably the IsoView SPIM, the Lattice Light Sheet Microscope and swept confocally aligned planar excitation. These technologies overcome the limitations of conventional optical sectioning techniques and enable unprecedented levels of spatio‐temporal resolution with low levels of phototoxicity. Developing in parallel with powerful computational approaches, light sheet based methods promise to completely transform cell biology as we know it today.