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In vitro tracking and intracellular protein distribution in immunology
Author(s) -
Zibaei Kajal,
Russell Sarah M
Publication year - 2017
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.2017.29
Subject(s) - immune system , computational biology , molecular imaging , biology , fluorescence lifetime imaging microscopy , neuroscience , microbiology and biotechnology , computer science , immunology , fluorescence , physics , in vivo , genetics , quantum mechanics
New imaging techniques have enabled major advances in understanding how immune reactions are initiated, coordinated and controlled. Imaging methods, which were previously mostly descriptive and supplementary to more quantitative approaches, have now reached sufficient precision and throughput that they are becoming integral to almost all aspects of immunology research. Imaging methodologies that increase the resolution and sensitivity of detection, alongside an ever‐expanding range of fluorescent reporters of molecular and cellular activity, and vastly improved analysis methods, have all facilitated this transformation. In this review, we will discuss how advances in imaging are changing the way we view immune activation and control using T cells as the model immune system. We will describe how imaging has transformed our knowledge of molecular and signalling events in T‐cell activation, and the impact of these molecular events on the behaviour of T cells.