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Annexin A6 regulates interleukin‐2‐mediated T‐cell proliferation
Author(s) -
Cornely Rhea,
Pollock Abigail H,
Rentero Carles,
Norris Sarah E,
AlvarezGuaita Anna,
Grewal Thomas,
Mitchell Todd,
Enrich Carlos,
Moss Stephen E,
Parton Robert G,
Rossy Jérémie,
Gaus Katharina
Publication year - 2016
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.2016.15
Subject(s) - microbiology and biotechnology , cell growth , annexin a2 , interleukin , interleukin 15 , annexin , immunology , biology , cytokine , flow cytometry , genetics
Annexin A6 (AnxA6) has been implicated in cell signalling by contributing to the organisation of the plasma membrane. Here we examined whether AnxA6 regulates signalling and proliferation in T cells. We used a contact hypersensitivity model to immune challenge wild‐type (WT) and AnxA6 −/− mice and found that the in vivo proliferation of CD4 + T cells, but not CD8 + T cells, was impaired in AnxA6 −/− relative to WT mice. However, T‐cell migration and signalling through the T‐cell receptor ex vivo was similar between T cells isolated from AnxA6 −/− and WT mice. In contrast, interleukin‐2 (IL‐2) signalling was reduced in AnxA6 −/− compared with WT T cells. Further, AnxA6‐deficient T cells had reduced membrane order and cholesterol levels. Taken together, our data suggest that AnxA6 regulates IL‐2 homeostasis and sensitivity in T cells by sustaining a lipid raft‐like membrane environment.