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miR‐34a inhibits the apoptosis of MDSCs by suppressing the expression of N‐myc
Author(s) -
Chen Si,
Huang Anfei,
Chen Huo,
Yang Yi,
Xia Fei,
Jin Liping,
Zhang Jinping
Publication year - 2016
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.2016.11
Subject(s) - cancer research , microrna , apoptosis , myeloid derived suppressor cell , population , suppressor , bone marrow , spleen , chemistry , immunology , biology , medicine , cancer , gene , biochemistry , genetics , environmental health
Myeloid‐derived suppressor cells (MDSCs) are a heterogeneous population of early myeloid progenitors, which possess strong immunosuppressive functions. MDSCs are found in increased numbers in infectious and inflammatory pathological conditions. However, whether microRNAs have a role in the expansion remains unclear. Here in our study, we found that overexpression of miR‐34a could induce the expansion of MDSCs in the bone marrow and spleen both in chimera and transgenic mice. And further experiments demonstrated that miR‐34a inhibited the apoptosis through reduced translation of N‐myc without affecting the proliferation. Luciferase assay and western blotting experiments implied that N‐myc is the direct target of miR‐34a in MDSCs. Overexpressed mir‐34a changes the cytokine expression profile in MDSCs and skewed the MDSCs to M1 phenotype. And miR‐34a‐overexpressed MDSCs significantly slowed down the tumor growth. Taken together, miR‐34a contributes to the expansion of MDSCs by inhibiting the apoptosis via suppressing the expression of N‐myc.