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Naive CD8 + T‐cell precursors display structured TCR repertoires and composite antigen‐driven selection dynamics
Author(s) -
Neller Michelle A,
Ladell Kristin,
McLaren James E,
Matthews Katherine K,
Gostick Emma,
Pentier Johanne M,
Dolton Garry,
Schauenburg Andrea JA,
Koning Dan,
Fontaine Costa Ana Isabel CA,
Watkins Thomas S,
Venturi Vanessa,
Smith Corey,
Khanna Rajiv,
Miners Kelly,
Clement Mathew,
Wooldridge Linda,
Cole David K,
Baarle Debbie,
Sewell Andrew K,
Burrows Scott R,
Price David A,
Miles John J
Publication year - 2015
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.2015.17
Subject(s) - repertoire , biology , t cell receptor , cd8 , antigen , immune system , t cell , immunology , acoustics , physics
Basic parameters of the naive antigen (Ag)‐specific T‐cell repertoire in humans remain poorly defined. Systematic characterization of this ‘ground state’ immunity in comparison with memory will allow a better understanding of clonal selection during immune challenge. Here, we used high‐definition cell isolation from umbilical cord blood samples to establish the baseline frequency, phenotype and T‐cell antigen receptor (TCR) repertoire of CD8 + T‐cell precursor populations specific for a range of viral and self‐derived Ags. Across the board, these precursor populations were phenotypically naive and occurred with hierarchical frequencies clustered by Ag specificity. The corresponding patterns of TCR architecture were highly ordered and displayed partial overlap with adult memory, indicating biased structuring of the T‐cell repertoire during Ag‐driven selection. Collectively, these results provide new insights into the complex nature and dynamics of the naive T‐cell compartment.