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CD244 is expressed on dendritic cells and regulates their functions
Author(s) -
Georgoudaki AnnaMaria,
Khodabandeh Sorosh,
Puiac Speranta,
Persson Catrine M,
Larsson Maria K,
Lind Max,
Hammarfjord Oscar,
Nabatti Tara H,
Wallin Robert P A,
Yrlid Ulf,
Rhen Mikael,
Kumar Vinay,
Chambers Benedict J
Publication year - 2015
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.2014.124
Subject(s) - biology , receptor , dendritic cell , immunology , cd86 , microbiology and biotechnology , immune system , cd80 , t cell , cd40 , in vitro , cytotoxic t cell , biochemistry
Signaling lymphocytic activation molecule (SLAM) receptors have an important role in the development of immune responses because of their roles, for exampe, in NK cell cytotoxicity and cytokine production by NK, T cells and myeloid cells. The SLAM receptor CD244 (2B4, SLAMf4) is expressed on a variety of immune cell types but most of its functions have been examined on NK and T cells. In the present study, we investigated expression and function of CD244 in murine subsets of dendritic cells (DCs). We report that all subsets of murine DCs examined expressed CD244, although the expression levels of CD244 varied between subsets. Splenic and resident mesenteric lymph node (MLN) DCs from CD244 −/− mice expressed lower levels of CD86 and MHC class II compared with wild‐type mice. Upon Toll‐like receptor (TLR) stimulation, no differences in surface expression of these molecules were observed between DCs from CD244 −/− and wild‐type mice. However, splenic DCs from CD244 −/− mice upon stimulation with TLR binding ligands lipopolysaccharide (LPS) and CpG produced significantly higher levels of pro‐inflammatory cytokines. In addition, DCs from CD244 −/− mice elicited increased NK cell activation in vitro . These data add CD244 to a growing list of immuno‐modulatory receptors found on DCs.