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Innate interferons inhibit allergen and microbial specific T H 2 responses
Author(s) -
Pritchard Antonia L,
White Olivia J,
Burel Julie G,
Upham John W
Publication year - 2012
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.2012.39
Subject(s) - immunology , cytokine , allergen , biology , antigen , peripheral blood mononuclear cell , toxoid , house dust mite , interferon , allergy , virology , immunization , in vitro , biochemistry
Several studies provided evidence of innate interferons (IFNs) regulating T H 2 cytokine production using purified CD4 + memory cells and T H 2 polarisation via interleukin‐4 (IL‐4). Vitally, none of these previous studies examined IFN attenuation of T H 2 responses to allergen or antigen. This study therefore sought to investigate the abrogation of specific allergen‐ and antigen‐stimulated T H 2 response in peripheral blood mononuclear cells (PBMC) derived from 12 sensitised individuals by IFN‐β and IFN‐λ. PBMC were cultured in the presence of house dust mite (HDM) allergen, rhinovirus (RV), influenza vaccine and tetanus toxoid (TT)±either IFN‐β or IFN‐λ for 3 and 5 days. IFN‐γ, IL‐5 and IL‐13 protein levels were measured by ELISA. Quantitative PCR (qPCR) was used to investigate induction of genes involved in control of T H 2 cytokines. No alteration in T H 1 IFN‐γ allergen/antigen response was observed with addition of IFN‐β or IFN‐λ. Consistent abrogation of T H 2 response to HDM and influenza was observed with IFN‐β at both time points; attenuation was observed by day 5 with RV and TT. IFN‐λ had no consistent effect on T H 2 production except in the presence of RV (multiplicity of infection=5); a decrease in IL‐5 alone was observed in the presence of trivalent inactivated influenza vaccine. GATA binding protein 3 (GATA3) and suppressors of cytokine signalling3 mRNA were differentially regulated in HDM and influenza‐stimulated cultures±IFN‐β. We concluded that IFN‐β produced a strong and consistent abrogation of T H 2 cytokine production in the presence of a range of allergen and antigen stimulants.