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Tumor necrosis factor negative bone marrow‐derived dendritic cells exhibit deficient IL‐10 expression
Author(s) -
Roomberg Alicia,
Kling Jessica,
Fromm Phillip,
Körner Heinrich
Publication year - 2010
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.2010.54
Subject(s) - tumor necrosis factor alpha , receptor , microbiology and biotechnology , effector , bone marrow , biology , immunology , dendritic cell , innate immune system , chemistry , immune system , biochemistry
The effective maturation of dendritic cells (DC) is complex and highly regulated and requires the presence of a variety of signals. Tumor necrosis factor (TNF) and its receptors or innate pattern recognition receptors such as the toll‐like receptors have been shown to contribute to this process. DC derived from bone marrow cells in the presence of granulocyte/macrophage colony‐stimulating factor can be used as a model to ascertain the contribution of different signals to DC maturation. Analysis of DC activated by addition of the mycobacterial vaccine strain Bacillus Calmette‐Guérin showed that of the effector molecules studied only interleukin‐10 expression was significantly reduced in TNF‐negative (B6.TNF −/− ) DC. Another effector molecule produced by DC, inducible nitric oxide synthase, was largely unchanged.