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Myelopoiesis related to perinatal spleen
Author(s) -
Hinton Rebecca,
Petvises Sawang,
O'Neill Helen
Publication year - 2010
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.2010.156
Subject(s) - spleen , myelopoiesis , biology , immunology , progenitor cell , stromal cell , bone marrow , myeloid , dendritic cell , haematopoiesis , embryonic stem cell , stem cell , microbiology and biotechnology , immune system , cancer research , genetics , gene
Adult murine spleen is known to have a major role in the development of dendritic cell (DC) subsets, including conventional DC and plasmacytoid DC. In this lab, long‐term cultures (LTCs) established from murine spleen support continuous production of novel dendritic‐like cells, termed LTC‐DC. An in vivo equivalent subset also exists in spleen, namely L‐DC. As co‐cultures using LTC‐derived splenic stroma support the outgrowth of L‐DC from spleen and bone marrow sources, it is likely that spleen represents an important niche for DC development. To investigate the appearance of L‐DC during ontogeny, spleen was isolated from embryonic and neonatal mice of different ages for analysis of myeloid and DC subsets. Perinatal spleen was also used to establish co‐cultures for identification of progenitors, and LTCs were established from spleens for assessment of stromal competence. Although spleen from 16‐day embryos (E16.5) contained myeloid cells, DC subsets did not appear until day 4 after birth (D4). However, murine spleen at D0 contained progenitors, which could seed co‐cultures for L‐DC production. LTC could not be established from spleen until D4. The appearance of L‐DC after D4 in spleen is dependent on the formation of the appropriate stromal microenvironment which occurs in the early postnatal period.