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A lipopeptide based on the M2 and HA proteins of influenza A viruses induces protective antibody
Author(s) -
Pejoski David,
Zeng Weiguang,
Rockman Steven,
Brown Lorena E,
Jackson David C
Publication year - 2010
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.2010.15
Subject(s) - lipopeptide , ectodomain , virology , epitope , hemagglutinin (influenza) , virus , biology , antibody , peptide sequence , influenza a virus , peptide vaccine , peptide , immunodominance , microbiology and biotechnology , immunology , biochemistry , receptor , bacteria , genetics , gene
A conserved 15 amino‐acid residue sequence of the ectodomain of the M2 protein of influenza A virus (M2e) induces a strong antibody (Ab) response when incorporated into a synthetic lipopeptide vaccine candidate containing a T‐helper epitope from influenza A hemagglutinin and the dendritic cell‐targeting lipid moiety S‐[2,3‐bis(palmitoyloxy)propyl]cysteine (Pam2Cys). Abs elicited by the truncated M2e sequence were specific for the M2 protein of influenza A virus and were also capable of binding to cells that were infected with influenza A viruses of different subtypes. The Ab titres against the lipopeptide were similar in magnitude to those elicited by the full‐length (23 residue) M2e peptide when administered in Freund's adjuvant. Abs to the truncated M2e sequence were also able to significantly reduce the viral load in airways of BALB/c mice after challenge with live influenza virus.