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Interleukin 10 decreases MICA expression on melanoma cell surface
Author(s) -
Serrano Antonio E,
MenaresCastillo Evelyn,
GarridoTapia Macarena,
Ribeiro Carolina Hager,
Hernández Carolina J,
MendozaNaranjo Ariadna,
GaticaAndrades Marcela,
ValenzuelaDiaz Rodrigo,
Zúñiga Roberto,
López Mercedes N,
SalazarOnfray Flavio,
Aguillón JuanCarlos,
Molina MaríaCarmen
Publication year - 2011
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.2010.100
Subject(s) - nkg2d , mhc class i , cytotoxic t cell , major histocompatibility complex , natural killer cell , cell , lymphokine activated killer cell , microbiology and biotechnology , cell culture , biology , chemistry , cytotoxicity , immune system , interleukin 12 , cancer research , immunology , in vitro , biochemistry , genetics
Natural‐killer group 2, member D (NKG2D) binds to a variety of ligands, including the major histocompatibility complex (MHC) class I chain‐related proteins (MIC) and UL16‐binding proteins (ULBP). It is regarded as a co‐activating receptor on NK cells, having an important role in the cell‐mediated immune response to tumours. We studied the influence of interleukin (IL)‐10 on the regulation of MIC and ULBP expression on melanoma cells, and its effect on the cytotoxic function of NK cells in vitro . Here, we show that, in the presence of IL‐10, FMS mel and BL mel cell lines decreased MICA and ULBP2 surface expression, whereas MHC class I did not change substantially on the cell surface. MICA mRNA levels decreased in IL‐10‐treated FMS and IL‐10‐transduced BL cell lines. Interestingly, we observed that MICB surface expression and its mRNA levels increased upon IL‐10 treatment in a melanoma cell line. These changes in NKG2D ligands surface expression patterns owing to IL‐10 treatment resulted in an effect on lysis susceptibility mediated by lymphocyte‐activated killer cells, as tumour cell lines that displayed a higher decrease of MICA on their surface had lower levels of lysis. In addition, expression of CD107a was downregulated on the surface of NK cells following stimulation with IL‐10‐treated FMS cells. Our results suggest a novel function for IL‐10 in the modulation of NKG2D ligand expression and in the control of cytotoxicity mediated by NKG2D/NKG2D ligand axis.