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Role of NADPH oxidase‐2 in lipopolysaccharide‐induced matrix metalloproteinase expression and cell migration
Author(s) -
Kim SoYeon,
Lee JinGu,
Cho WooSung,
Cho KyongHyun,
Sakong Jun,
Kim JaeRyong,
Chin ByungRho,
Baek SukHwan
Publication year - 2010
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.2009.87
Subject(s) - apocynin , nox1 , nadph oxidase , small interfering rna , reactive oxygen species , matrix metalloproteinase , chemistry , nox4 , microbiology and biotechnology , cell migration , lipopolysaccharide , mapk/erk pathway , signal transduction , cell , biology , biochemistry , immunology , transfection , gene
This study examined the hypothesis that the control of NADPH oxidase‐2 (Nox2)‐mediated reactive oxygen species (ROS) regulates the expression of matrix metalloproteinases (MMPs) and the migration of macrophages. Lipopolysaccharide (LPS) stimulation of Raw264.7 cells and mice peritoneal macrophages increased the expression of MMP‐9, 10, 12 and 13 mRNA, and also increased Raw264.7 cell migration. Treatment with an antioxidant ( N ‐acetyl cysteine) or Nox inhibitors strongly inhibited the expression of MMPs by LPS and inhibited cell migration. LPS caused ROS production in macrophages and increased the mRNA expression of Nox isoforms Nox1 and Nox2 by 20‐fold and two‐fold, respectively. While Nox1 small interfering RNA (siRNA) did not inhibit LPS‐mediated expression of MMPs, Nox2 siRNA inhibited the expressions of MMP‐9, 10 and 12. Neither Nox1 nor Nox2 siRNA influenced the LPS‐mediated expression of MMP‐13. In addition, NAC or apocynin attenuated LPS‐induced ROS production and MMP‐9 expression. MMP‐9 expression and cell migration were controlled by ERK1/2–ROS signaling. Collectively, these results suggest that LPS stimulates ROS production via ERK and induce various types of MMPs expression and cell migration.