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Predicting CD62L expression during the CD8 + T‐cell response in vivo
Author(s) -
Schlub Timothy E,
Badovinac Vladimir P,
Sabel Jaime T,
Harty John T,
Davenport Miles P
Publication year - 2010
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.2009.80
Subject(s) - adoptive cell transfer , cd8 , cytotoxic t cell , biology , t cell , t cell receptor , microbiology and biotechnology , cell division , immunology , immune system , cell , in vitro , genetics
Acute infection leads to CD8 + T‐cell activation, division and differentiation. Following clearance of infection, cells revert to two distinct subsets of memory, central (T CM ) and effector (T EM ) memory. Adoptive transfer of naive T‐cell receptor transgenic (TCR‐tg) T cells has been used to study the differentiation of these memory subsets, which are often discriminated by expression of CD62L. Naive CD8 + T cells are CD62L high , and CD62L expression is lost during the ‘effector’ phase. Adoptive transfer studies show that higher transfer frequencies result in diminished T‐cell expansion and a higher proportion CD62L high . This suggests a relationship between CD62L expression and cell division, where division leads to conversion from CD62L high to CD62L low phenotype. To address this hypothesis we adoptively transferred graded numbers of OT‐1 TCR‐tg T cells from naive donors and tracked the kinetics and phenotype of the immune response after infection. We developed a simple mathematical model of division‐linked CD62L differentiation, which we compared with the experimental data. Our results show that division‐linked differentiation predicts the differences in proportion of cells CD62L high observed between responses of different adoptive transfer number and within individual mice. We calculate that approximately 20% of CD62L high cells convert to CD62L low during each division.