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Monoclonal antibodies generated by DNA immunization recognize CD2 from a broad range of primates
Author(s) -
Brady Jamie L,
Mannering Stuart I,
Kireta Svjetlana,
Coates Patrick T,
Proietto Anna I,
Cowan Peter J,
D'Apice Anthony J F,
Lew Andrew M
Publication year - 2009
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.2009.4
Subject(s) - monoclonal antibody , toxoid , immunization , biology , immunology , baboon , antibody , nod , virology , xenotransplantation , peripheral blood mononuclear cell , heterologous , microbiology and biotechnology , in vitro , in vivo , medicine , transplantation , gene , genetics , surgery , endocrinology
Using heterologous prime‐boost (DNA immunization followed by immunization with transfected cells), we have generated depleting mouse anti‐baboon CD2 monoclonal antibodies (mAb). These anti‐CD2 mAb recognized a diverse range of primate CD2 from New World monkeys and Old World monkeys to humans and have potent immunosuppressive activity for human allo‐MLR responses and anti‐tetanus‐toxoid recall responses. There was no upregulation of activation markers or release of cytokines when the mAb were incubated with human peripheral blood mononuclear cells. Using chimeric NOD‐SCID IL2rγ null mice, the mAb were shown to deplete human and cynomolgus monkey T cells in vivo . These anti‐CD2 mAb may therefore be important immunological tools in allo‐ and xenotransplantation.