Premium
Ontogeny and phagocytic function of baboon lung dendritic cells
Author(s) -
Awasthi Shanjana,
Wolf Roman,
White Gary
Publication year - 2009
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.2009.2
Subject(s) - baboon , cd86 , biology , flow cytometry , cd11c , dendritic cell , immunology , lung , cd80 , fetus , population , cd40 , andrology , immune system , microbiology and biotechnology , pathology , t cell , medicine , endocrinology , cytotoxic t cell , in vitro , pregnancy , biochemistry , genetics , environmental health , gene , phenotype
Dendritic cells (DCs) are the most potent antigen‐presenting cells, but the ontogeny and functions of lung DCs are not known during prenatal period. Here, we isolated lung DC population from fetal (125–175 days of gestation age) and adult baboons. The cells were stained with fluorochrome‐conjugated‐HLA‐DP, DQ, DR, CD1a, CD11c, CD14, CD40, CD80, CD86, CD209, CMKLR1, ILT7‐specific antibodies, and staining was analyzed by flow cytometry. The phagocytic function was investigated by incubating the cells with fluorescent‐labeled Escherichia coli bioparticles and analyzed by flow cytometry and fluorescence microscopy. The fetal baboon lung DCs expressed low levels of HLA‐DP, DQ, DR, CD11c and CD86 as compared to adult baboon lung DCs and showed distinct DC morphology. The fetal lung DCs were also less capable of phagocytosing E. coli as compared to the adult lung DCs ( P <0.05). In conclusion, the fetal lung DCs are not only phenotypically immature, but also less efficient in phagocytosing E. coli .