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The cellular mechanism by which complementary Id + and anti‐Id antibodies communicate: T cells integrated into idiotypic regulation
Author(s) -
Jacobsen Johanne T,
Lunde Elin,
SundvoldGjerstad Vibeke,
Munthe Ludvig A,
Bogen Bjarne
Publication year - 2010
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.2009.118
Subject(s) - antibody , biology , epitope , antigen , idiotopes , b cell , immunoglobulin d , microbiology and biotechnology , mechanism (biology) , immunology , idiotype , monoclonal antibody , philosophy , epistemology
The V region antigenic determinants (idiotopes (Ids)) of antibodies (Abs) have been suggested to be involved in regulating the immune system. Certain diseases such as diabetes mellitus have recently been associated with a disequilibrium between Id + and anti‐Id Abs. However, it is unknown how Abs carrying complementary idiotypes (that is, Id + and anti‐Id Abs) regulate each other at the level of B and T cells. In this study, we show that B lymphoma cells genetically equipped with anti‐Id BCR V regions receive a signal when exposed to Id + Ig. Moreover, they become × 10 4 more efficient at presenting exogenous Id + Ab to CD4 + T cells in vitro . Activated Id‐specific T cells in turn regulated the Id‐specific B lymphoma cells. Similar results were obtained in vivo in a surrogate model in which an Id‐peptide was incorporated genetically into the C‐region of a recombinant Ab that targeted IgD on B cells. The findings suggest that conventional T–B collaboration can explain communication between complementary Id + and anti‐Id Ab at the cellular level. A model is suggested that integrates present and previous data on B‐cell regulation by Id‐specific T cells.